Nested PCR definition of species composition of malaria parasites in Thanh commune, Huong Hoa district of Quang Tri province

Nested polymerase chain reaction (nested PCR) was applied to idennify species composition of four human malaria parasite species in Thanh commune, Huong Hoa district of Quang Tri province. The pair of primers specific to each species of P.falciparum, P.vivax, P.malariae and P.ovale were used. AND fragments specific to each species was 18ssr-RNA)[5], [10]. The analysis of 152 blood samples showed only two species of malaria parasites of P.falciparum (71%) and P.vivax (29%) present in the study area. Single P.falciparum infection rate was 65.8%, single P.vivax infection rate was 16.4% and the double infection of P.falciparum and P.vivax was 17.8%

Study of prevalence of mixed species malaria infection and distinguishment of recrudescence from re-infection in Gia Lai province by the use of nested polymerase chain reaction (PCR)

Application of PCR technique to survey component and mechanism of 4 human malaria parasites in Gia Lai province. These used bait couples were specificity for P.falciparum, P.vivax, P.malariae and P.ovale. The results showed that: there were the existance of 4 human malaria parasite species, of which P.falciparum was 57.6%, combination rate of 2,3,4 parasites species was 54.76%. To distinguish the recurrent, re-infection based on the difference of alleles in locus gen. 3 genes for highly polymorphic antigens: merozoite surface protein 1 (MSP1), merozoite surface protein 2 (MSP2), glutamate rich protein (GLURP). 11 patients were treated by fansidar had been recurrented after 14 days